However, the observed superior activity of the cells expressing CYP1A1 2C I462V for hydroxylations of AF could not be explained by differential enzyme expression or inducibility because a real time RT PCR and Western blot did not detect any apparent differences in mRNA and protein expression levels of CYP1A1 among the selected stable clones expressing CYP1A1 1, CYP1A1 2C, and CYP1A1 4 Fig <a href=https://cialiss.skin>cialis without prescription</a> The principal metabolites identified are phosphoramide mustard, and acrolein